Human anti-Q fever antibody (anti-Q-Ab) ELISA Kit

Principle of test
The kit assay Human anti-Q-Ab level in the sample,use Purified Human anti-Q-Ab antibody to coat microtiter plate wells, make solid-phase antibody, then add anti-Q-Ab to wells, Combined anti-Q-Ab antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of anti-Q-Ab in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human anti-Q fever antibody (anti-Q-Ab) ELISA Kit