Human Deoxypyridinoline crosslinks (DPD) ELISA Kit

The kit assay Human DPD level in the sample,use Purified Human DPD antibody to coat microtiter plate wells, make solid-phase antibody, then add DPD to wells, Combined DPD which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DPD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Deoxypyridinoline crosslinks (DPD) ELISA Kit