Human Interleukin 17 (IL-17) ELISA Kit

Purpose
This kit allows for the determination of IL-17 concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human IL-17 level in the sample,use Purified Human IL-17 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-17 to wells, Combined antibody which With HRP labeled goat anti-Human become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-17 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Interleukin 17 (IL-17) ELISA Kit