Rat nuclear factor Kappa B (NF?b) ELISA Kit

Purpose
This kit allows for the determination of NF?b concentrations in Rat serum, tissue and other biological fluids.
Principle of the assay
The kit assay Rat NF?b level in the sample,use Purified Rat NF?b to coat microtiter plate wells, make solid-phase antibody, then add NF?b to wells, Combined NF?b antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NF?b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Rat nuclear factor Kappa B (NF?b) ELISA Kit